Vertebrate
Development... Embryology:
Campbell 11e readings
- c46
p1026-1027; c47
p1037-1055
Campbell 10e readings -
c46 (1014-16; 1022-23; 1030; c47
p1037-1051)
Embryology
-
study of development of the embryo
5
major stages... [gametogenesis,
fertilization, clevage, gastrulation, and organogenesis]
1.
gametogenesis
- gamete production - (i.e.,
MEIOSIS)
spermatogenesis
-
in
semiferous*
tubule
spermatocytes
- undergo meiosis 1 & 2 --> sperm
cells
characterized by "cell differentiation"
oogenesis
- in
ovary*
-
1st
meiotic division is
unequal*
-
snail
egg cells
every
28d FSH (of pituitary)
stimulates dormant follicle
to develope
oocytes
--meiosis--> produce 2nd oocyte &
polar body
LH
(pituitary) triggers ovulation
characterized by "cell growth -
enlargement"
Comparison
figure
of gametogenic
cell divisions
2. Fertilization
- union of sperm & egg cell --> 2n zygote...
[forms
recombinant DNA]
parts*of
a sperm
- acrosome, head,
mito, & axoneme
(flagella)
"sperm
is no more than a delevery vehicle for male DNA,
a genome with a hyperactive
tail "
sperm must
penetrate*...
1) egg's outer, protective, jelly-like coat,
2) vitellin
layer... (glycoproteins), &
photo
by
Lennart Nilsson
3) egg's plasma membrane
via ACROSOME
REACTION*...
requires specificity
- (mammalian
version)*.
monospermy:
fusion of a single sperm and egg nuclei.
prevention
of polyspermy
(fusion by many sperm):
in sea urchins plasma membrane & vitellin layer
becomes depolarized, prevents
fusion;
also, Ca++ is released from egg's E.R. and a
wave of Ca*
sweeps across egg... Video*
high Ca++
leads to
cortical reaction*
creates perivitellin space as
plasma membrane lifts
due to
hyalin release -
a glycoprotein that is attracts water to space.
after
fert, area hardens and separates... forming
fertilization membrane*
high
Ca also leads to activation of egg = cell
respiration & protein
synthesis
timeline of events*
3.
Cleavage*
- rapid succession of cell
divisions... without cell growth
-
no increase in size, only an increase in cell
numbers - fig*
(pics echinoderm* - starfish-4-cell -
invert* -
invert-2 -
SEM-frog*
- Human*
)
forms hollow ball of cells i.e., BLASTULA embryo
(video*)
outermost blastomeres
join via tight junctions & seal interior cell mass,
whose cells
pump Na
into cavity favoring osmotic gradient into internal fluid
cavity
called the... blastocoel.
mouse
blastula w stem cells & Human
postfertilization events
in
mammals
Blastocyst
(or
trophoblast) will
become the placenta,
while
the inner
mass of cells*
will form the embryo proper.
General
clevage pattern
pics
sea urchin cleavage to blastula embryo*
(video)
model animal = round worm (C. elegans)
(video1
video2)
depends frog*
&
pics
frog cleavage*
upon
yolk chick*
&
pics
chick cleavage
animal
pole
- is portion of embryo housing primary
tissues
vegetal pole
- is portion of embryo containing "yolk"
- that establish axes
3
day old human embryo (8 cell stage) &
injection
of DNA to modify genome
manipulation
of human blastula embryo
& animation
- Human embryonic stem cells
Embryogenesis
& Germ Cell Layers
4.
Gastrulation
- period of cell migrations
around blastopore
(an
organizing center)...
an opening into archenteron controlling cell migrations,
that converts embryo from hollow ball of cells into
a 3 layered stage called
gastrula embryo*
& pic
forms 3 primary germ cell layers
:
ectoderm - outer epidermal
layers of organs, skin --> epithelial
& nerve
endoderm - digestive tract
tissue --> gut
& endocrine glands
mesoderm - fills in space in
between --> notochord, muscle
& connective
(invert*
video*)
& (frog*
video )
& (chick*)
gastrulation
obliterates the blastocoel
- forms new
cavity called archenteron -
presumptive digestive cavity
►
period when new
diploid genes of embryo
becomes active
5.
Organogenesis... Organ
Formation via -
organizing centers*
groups
of cells that control fate of (organize) other cells
ex:
neuralation*
in frog - formation of
nervous system via neural tube
neural plate (ectoderm) - flat tissue surface
that migrates
via cytoskeletal elements to
to form tubes*
frog video
neural tube
= brain & spinal cord
notochord
(mesoderm) forms cartilage-like backbone
►
almost all
organs form
from flat plates
into tubes -->
3D shape
► following
a segmented development
pattern via
somites
____________________________________________________________________________________________________________________________________________________________________________________
Mouse embryo development: mouse
vasculature
Human
development:
Human fertilization-clevage
& Human
blastula-gastrula
5,
14 and 20week Human embryos
slide
show of Human development
PBS
video of Human development (with ad)
fish,
chick, pig, & human embryo development movies
(WGBH-Nova)
the
23 stages of human development (Visible
embryo & NIH)
the
multidimensional
human embryo - U of Michigan
Some key differences:
comparing
animal
to plant
development...
major
differences occur in mechanisms of...
1.
MORPHOGENEIS: the
developmental mechanisms establishing
SHAPE/FORM
and ORGANIZATION (body plan)
in
animals:
cell migrations
- where cells create new
morphogenic shapes
by
migrating cells forming plates &
tubes
& embryonic induction
- where one group of cells influences development
of
an adjacent group of cells
ex :
lens induction in eye
in
plants:
cytokinesis & plane
of Cell Division*
fig
21.4a &
fig 21.4b
2.
CELL DIFFERENTIATION:
processes by which cells becomes
SPECIALIZED in structure and
function
Genetic
Basis of Cell Differentiation
- is
Differential Gene Activity
cells become structurally, functionally, & biochemically
different
by expressing different
genes at different times in
development
Transcriptional Regulation:
results in expression of Tissue Specific Proteins -fig
18.10*
Do
all cells posses a "full genetic complement" to express
the whole organism???
must demonstrate all cells of an organism
contain a complete genetic
complement
i.e., differentiation does not proceed by loss of genes
with time
Genomic
Equivalency:
all cells contain same genes:
nuclear transplantation
experiments.
1. F.C. Steward (1950) using
carrots*
grows whole plant from single cell
2.
Briggs & King (1952) &
J.B. Gurdon (1962)
demonstrate totipotency in
frogs*
3. Ian Wilmut (1997) clones
Dolly
- cloning*
Clone
your Pet
4.
S. Yamanaka (2002)
4 genes can reprogram adult mouse cells to pluripotent
cells.
Thus cells possess a complete genome to
express whole organism:
Genomic Equivalency
Stem Cells:
unspecialized cells (in form & shape) that can
reproduce indefinitely
under appropriate conditions --> differentiate into one
or more cell types
fertilized egg cells are totipotent
,i.e., all are embryonic stem cells
vs. adult stem cells (pluripotent
= many, but not all)-
stem cells*
key
concepts*
back
copyright
c2019
next
Charles
Mallery, Biology
150, Department of Biology, U. of Miami, Coral
Gables, FL
33124
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