Respiratory enzymes and coenzymes in eukaryote mitochondria. ...electrons flow from more negative toward more positive... (isolate mitochondrial membrane proteins and determine their E'o)

The redox potential of samples are determined in a cuvette with an electrometer through an appropriate reference electrode. The system is kept anaerobic (to avoid electron transfer to O2) by flowing N2, helium, or argon over the reaction mixture.      BACK