An advantage of an Eadie-Hofstee plot over a Lineweaver Burk plot is that the Eadie-Hofstee plot does not require a long extrapolation to calculate Km.
| Eadie
Hofstee
Plot (G.S.
Eadie (Duke U.) & B.H.J. Hofstee (Palo ALto
Res. Foundation) |
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| The Eadie-Hofstee plot is a way of plotting kinetic enzyme data so as to yield a straight line for reactions obeying Michaelis-Menten kinetics. This is done by plotting reaction velocity (V) versus velocity/substrate concentration (V/[S]). The slope of the line is equal to -KM and the y-intercept is Vmax. | |
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