a Tensegrity Model of Cell by Donald
Ingber see-
The Architecture of Life
by D. Ingber
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on flexible-puckering surface -
rounded nucleus
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on
rigid surface - flattened nucleus
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tissue culture cells
above monolayer have rounded nuclei |
- monolayer of cells in
tissue culture are flattened |
back
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Ingber's view of
the cell as a tensegrity structure may help explain why cells
in tissue culture spread out and flatten when grown on rigid
glass of plastic petri dishes, but when on a flexible surface
the cells contract and become spherical.
-
- Ingber modeled a cell
as a tensegrity structure made of six wood dowels and elastic
string. The wooden dowels occurred in three pairs, each
perpendicular to the other two and bore the compressive
stress. Tension bearing elastic string connected to the ends
of the dowels, pulling them into a stable, 3-D form. A
smaller, spherical tensegrity model, inside the larger one,
represented the
- cell's nucleus. Stretched elastic
strings between the nucleus and dowels mimicked
cytoskeletal connections.
-
- Applying downward
force on the tensegrity cell model shapes it into a flattened
pile of sticks and string. When the force is removed, the
energy stored in the tensed filaments causes the cell model to
spring back to its original, roughly spherical shape. The
pictures above from Ingber's article's
suggest how cells may behave when placed on a rigid or
flexible surfaces.
-
- When
attached to a taut, stretched piece of cloth the model
flattens and spread out. The model's attachment to the flat
cloth is analogous to the adhesion receptors (integrins) which physically connect
a cell to an anchoring basement membrane substratum.
When the basement cloth is freed
from its moorings, thereby making the cell's anchoring surface
flexible, the tensegrity model rounds up into its more spherical
form, puckering the cloth underneath. The cell's nucleus
patterns its shape after that of the tensegrity model in a
coordinated fashion. In living cells nuclei spread and flatten
out in a similar manner when they adhere to a substrate.
Ingber's dowel and elastic string model of a cell, reveals that
tensegrity structures may mimic the known behavior of living
cells.
Tensegrity Model Structures:
straw models - Camp Elsewhere
- Domes
References :
Donald E.
Ingber - The Architecture of Life, Scientific
American, January 1998
Donald E. Ingber et al - Cellular Tensegrity
and Mechanochemical Transduction,
Annual
Reviews of Physiology, 1997, 19:329-339
Donald E. Ingber - Tensegrity: The Architectural Basis of
Cellular Mechanotransduction,
Annual Review of Physiology,
Vol. 59, pages 575--599; 1997.
- Donald E. Ingber, M.D. and Ph.D.
associate professor of pathology at Harvard Medical School and
a research associate departments of surgery Children's
Hospital in Boston. Professor Ingber is a founder of Molecular
Geodesics, a company which creates advanced materials based
upon biologically properties.