RECOMBINANT DNA FOUNDERS |
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ECO-R1 was 1st isolated by Herb
Boyer at UCSF in the fall of 1972. While attending a
conference in Hawaii Boyer, Cohen
and Falkow and having a
sandwich at a Waikiki Deli scribbled out on paper napkins
an experiment to use ECO-R1 to manipulate a bacterial
plasmid and make a recombinant DNA plasmid.
Many have said that this
moment was the origins of a commercial biotech industrial
revolution.
Within 5 months Boyer and Cohen used ECO-R1 to put a DNA piece, that imparted bacterial antibiotic resistance, into a plasmid (pSC101) and when introduced into E.Coli enabled resistance to that antibiotic. In 1973 John Morrow of Paul Berg's lab at Stanford using the protocols of Cohen and Boyer put frog rDNA into a plasmid and the plasmid was able to make rRNA in bacterial cells, establishing that the techniques would work with vertebrate DNA as well. |
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A global debate about the
biohazards of combining DNAs and the fears of creating
freakish organisms lead to the Asilonmar Conference in
February 1975... a debate that still rages to this day.
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In 1976
Bob Swanson an MIT graduate meets with Herb Boyer at UCSF
and they discuss the possibility of making insulin via
recombinant DNA technology and about forming a company to do
so. Swanson pitches the concept to capital investors and on
April 7th a new company is formed - GENENTECH
- based upon a patent that Stanford and UCSF had jointly
applied for in November of 1974 (the Boyer-Cohen cloning
methodology). On August 21, 1978 Boyer's group and Genentech
succeeded in making INSULIN in
bacteria using a synthetic DNA in a plasmid. |
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