DdeI is a restriction enzyme isolated from Desulfovibrio desulfuricans, a Gram-negative sulfate reducing soil bacteria) that can digest 1ug DNA in 1 hr @ 37C at cut site 5'C|TNAG3'                                                                                                                                3'GANT|C5'  The Sickle cell mutation is in the 6th codon and changes glutamic acid (+)  to    valine(0)         and effects the cut site.                                              5'-CTC-3'              to   5'-CAC-3'

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