1.0 ml of horse radish peroxidase homogenate has an Absorbance-A595 of 0.55 1.0 ml = 7.143 mg of albumin equivalent 1,400 ml = 7.143 x 1,400 = 10,000mg
1.0 ml converts 71.43
uMoles thus
7.143 mg protein converts 71.43 / 7.143 gives 10 Int'l
Units
10,000 mg protein =
100,000 IU
(1 I.U. is 1
uMole/min) |