Thiomargrita magnifica |
Cells of most bacterial species are around 2 µm in length.
Some members of sulfur-oxidizing gammaproteobacteria are known to reach up to 750 µm (average size: 180 µm). |
Using fluorescence, x-ray, and electron microscopy in
conjunction with genome sequencing, researchers at the
Genomic Institute of the Berkely National Lab
characterized Thiomargarita
magnifica, a bacterium with an average
cell length greater than 9,000 µm
that is visible to the naked eye
and is over the theoretical limits of bacterial size.
T.
magnifica grows as a white filament on
the decaying leaves in Caribbean mangroves in
Guadeloupe, Lesser Antilles. It is a single cell with a
thread-like body. On average, the T.
magnifica bacteria reaches up to 9,000 micrometers, with the
largest reaching the 2 centimeters and is visible with
the naked eye. The morphology of the T. magmifica filaments
observed from Guadeloupe resembled those of sessile
Thiomargarita-large sulfur bacteria-like cells reported
from deep-sea methane seeps. EM's of T.magnifica*.
Staining revealed DNA in T. magnifica cells was
concentrated in membrane-bound granules and not spread
throughout the cytoplasm, as is common in bacteria. No
bacteria or archaea are known to unambiguously segregate
their genetic material in the manner of eukaryotes,
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It has been assumed bacterial size was limited by
diffusional constants of molecules from bacterial walls
throughout its cytoplasm and waste products vice-versa.
This is likely overcome by T. magnifica’s large
internal water-filled sac that makes up some 73% of it
cell volume, so nutrients and waste don’t have to far to
travel. |
DNA sequencing show some 11 million base pairs and an
estimated 11,000 genes, 3 x
the size of most bacterial genomes. T.m's genome is highly
repetitive copies of some sequences reminiscent of more
complex life forms.
[Cold Spring Harbor’s bioRxiv, preprint]
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