STANDARD PROTEIN CURVE  
 
a protein reacts with a colored dye
   such as Biuret or Coomassie blue;
      color density is measured in a
          spectrophotometer
;
      units are absorbance at specific
           wavelength (here 595nm);
   

           absorbance is plotted
           vs. protein amount and          
  

   
  there is a linear relationship
 

       Sample Standard Curve*

dye bonding to proteins