RNA Polymerase DNA-directed RNA polymerase catalyzes the synthesis of RNA on a DNA template. The core enzyme has three polypeptide subunits: alpha, beta, & beta' in a stoichiometry of α2ββ' & an additional subunit, omega.  Core RNA polymerase can bind to DNA and catalyze the synthesis of RNA, but has no specificity, it needs a 5th subunit sigma factor to make it the holoenzyme, with specificity.

SUBUNITS:
 
 
alpha-
α - required for assembly of the enzyme;
                 interacts with some regulatory proteins;
                 also involved in catalysis.

  beta- β - involved in catalysis; chain initiation and elongation.

  beta prime - β' - binds to the DNA template.

  sigma - σ - directs enzyme to promoter, begins specific transcription.

  omega - ω - required to restore denatured RNA polymerase
                     in vitro to its fully functional form

RNA polymerase
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                polymerase core enzyme