Polynucleotide
Phosphorylase: |
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Polynucleotide PhosphorylaseAuthors: M. F. Symmons, G. H. Jones &
B. F. Luisi
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The channel through the PNPase has been suggested to function in the entrapment of the enzyme's single-stranded RNA substrate. The cores of each of the trimer subunits are formed from pairs of homologous domains colored here as the pairs violet and blue, blue-green and green, and finally orange and red respectively. | |
This view of the channel through the center of the trimer channel shows sidechains of the conserved 'FFRR loops' that may act as a mechanism to entrap RNA substrate and select against base-paired segments. This provides a possible structural explanation for both the high processivity and the regulation of the PNPase activity by RNA secondary structure. |